The Eastern Hellbender (Cryptobranchus alleganiensis alleganiensis) is a cryptic, long-lived, species in the family Cryptobranchidae. Declines in populations of many aquatic species, including C. a. alleganiensis may be related to changes in the streamside and watershed physical characteristics. This study examined the potential link between changes in substrate condition (fine sediment accumulation) and differences in C. a. alleganiensis length frequency, mass, tail circumference, and tail fin height within three tributaries to the Hiwassee River in North Carolina. Changes in these characteristics will indicate which habitats support the healthiest C. a. alleganiensis populations. I characterized the substrate within the three streams and sampled C. a. alleganiensis populations from the three streams. Snout-vent length frequencies were not significantly different among streams. Mean mass, mass:snout-vent length, and mass adjusted for snout-vent length in C. a. alleganiensis populations within Tusquitee Creek were larger when compared to Fires Creek, but not Brasstown Creek. The larger C. a. alleganiensis observed within Tusquitee Creek were thought to be the result of the compounding influences of stream reach position, sediment accumulation, point discharges, and other associated variables.